Five Hfr stains A through E are derived from a single F+ strain of E. coli. The following chart shows the entry time of the first five markers into an F- strain when each is used in an interrupted-conjugation experiment:
A: mal+ (1), str^s (11), ser+ (16), ade+ (36), his+ (51)
B: ade+ (13), his+ (28), gal+ (38), pro+ (44), met+ (70)
C: pro+ (3), met+ (29), xyl+(32), mal+ (37), str^s (47)
D: pro+ (10), gal+ (16), his+ (26), ade+ (41), ser+(61)
E: his+ (7), gal+ (17),pro+ (23), met+ (49), xyl+ (52)
a.) Draw a map of the F+ strain, indicating the positions of all genes and their distnaces apart in minutes.
b.) Show the intersection point in orientation of the F plasmid in each Hfr strain
c.) In the use of each of these Hfr strains, states which allele you would select to obtain the highest proportion of Hfr exconjugants